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Objective To investigate the inhibiting effect of acupuncture at points Neiguan(PC6) and Shuigou(GV26) on caspase-3, and PARP-1 mRNA and protein expressions in the cerebral cortex in rats with cerebral ischemia-reperfusion. Method Forty male SD rats were randomized to normal, sham operation, model and acupuncture groups. A model of middle cerebral artery infarction was made by modified Longa thread occlusion. Neurological function was scored using a 6-grade 5-point Bederson's scale. Caspase-3, and PARP-1 mRNA and protein expressions in rat cerebral cortex were determined using real-time PCR and Western Blot at 24 hrs after cerebral ischemia. Result Neurological deficit was reduced in the acupuncture group of rats with cerebral ischemia-reperfusion compared with the model group (P<0.01). Acupuncture could down-regulate caspase-3, and PARP-1 mRNA and protein expressions and there was a statistically significant difference compared with the model group (P<0.05, P<0.01; P<0.05, P<0.05). Conclusion Acupuncture at points Neiguan and Shuigou can down-regulate caspase-3, and PARP-1 mRNA and protein expressions in the cerebral cortex in rats with cerebral ischemia-reperfusion.
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Objective To investigate the protective effect of curcumin (Cur) on mice cornea against ultraviolet A (UVA)-induced damage.Methods Together 30 C57BL/6 mice were randomly divided into 3 groups (10 mice in each group):UVA irradiation group (UVA group),UVA irradiation with curcumin treatment group (UVA + Cur group) and control group.Mice in the UVA group was exposed to UVA for 24 h,with the wavelength of 320-400 nm and the irradiation intensity of 0.05 W · cm-2.In UVA + Cur group,mice were given 30 mg · kg-1 curcumin intraperitoneally 3 days before irradiation and lasted until 48 h after irradiation.And the control group was left untreated.Then corneal opacity was evaluated and scored through slit-lamp biomicroscopy.After irradiation for 48 h,corneal tissue was collected for the detection of the expression of NF-κB by electrophoretic mobility shift assay.Results After irradiation for 48 h,the score of corneal opacity in the UVA group (3.10 ± 0.74) was higher than that of the control group (0) and UVA + Cur group (0.35 ± 0.24) (P ＜ 0.05).The expression of NF-κB in cornea was detectable at a low level in the control group (1.25 ± 0.13),but was remarkably increased in UVA group (3.98 ± 0.58) and suppressed by curcumin in UVA +Cur group (1.58 ± 0.34) (P ＜ 0.05).There was no significant difference in corneal opacity score and NF-κB expression between control and UVA + Cur group (P ＞ 0.05),but there was significant difference between control and UVA group and UVA + Cur and UVA group (all P ＜ 0.05).Conclusion Curcumin can protect cornea against UVA-induced damage via inhibiting NF-κB activation.
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?AIM: To evaluate the effects on recovering of corneal wound and postoperative discomfort of different methods for primary pterygium.?METHODS: Forty-seven cases ( 60 eyes ) of primary pterygium were excised under microscope with limbal epithelial transplantation, with sharp dissection ( 24 cases, 30 eyes, Group A) and blunt dissection (23 cases, 30 eyes, Group B).All cases were followed up for 1d to 1mo.?RESULTS: The recovering of corneal wound was better in Group B on 1st day and 3rd day after surgery.Pain, photophobia and tears, foreign body sensation were more serious in group A on 1st day after surgery with a statistically significant difference (P=0.005,0.015,0.012). Pain, photophobia and tears, foreign body sensation were more serious in Group A on 3rd day after surgery with a statistically significant difference ( P=0.019,0.018, 0.015).There was no statistically significant difference on 1wk and 1mo after surgery (P>0.05).? CONCLUSION: Compared with sharp dissection, primary pterygium excised with blunt dissection can significantly improve recovering of corneal wound and postoperative discomfort.
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To evaluate the influence of phacoemulsification on corneal endothelium of senile cataract with lupus nephritis (LN). ●METHODS:This clinical trial involved 40 cataract patients with lupus nephritis (40 eyes), and 50 cases (50 eyes) without lupus nephritis. All of them underwent phacoemulsification+lOL implantation. The parameters of corneal endothelial cell including central corneal endothelium cell density ( CED ), average area of endothelial cell ( AVE) and coefficient of variation ( CV) were recorded by corneal endothelial microscope pre -operation and at one month after operation. The data were analyzed by SPSS 13. 0 statistical software. ● RESULTS: Both LN group and control group, the morphology of coneal endothelial was statistical significant differences between pre - operation and 1mo postoperation. The CED was lower, AVE and CV were higher ( P ● CONCLUSlON: The corneal endothelial cell in lupus nephritis patients is more fragile. Safe and reliable operation should be selected for these patients.